The Swift RNA Library Kit offers a fast, robust NGS transcriptomics workflow with optimal transcript coverage and NGS data quality from a broad range of input quantities for Illumina® sequencing platforms. Leveraging patented Adaptase® technology, this kit enables stranded RNA library construction directly from 1st strand cDNA without the requirement for 2nd strand cDNA synthesis and degradation, or template-switching methods. The kit is compatible with manual and automated workflows as well as upstream and downstream enrichment and depletion methods and supports a variety of indexing options.
Superior Data: High mapping rates, genes detected, and transcript coverage
Accommodates your Samples with a Single Kit: Consistent performance from 10 ng – 1 μg total RNA or 100 pg – 100 ng mRNA input
Robust Performance: Consistent libraries with minimal adapter dimers and no adapter titration required for all supported inputs
Save Time, Reduce Costs, Automate: Go from RNA to library in 4.5 hours; variety of index options including 96 UDIs and up to 768 combinatorial dual indexing; automation-friendly
Swift’s Adaptase technology allows for minimal adapter dimers with NO need for adapter titration. Compared to leading RNA library kits which can produce libraries with > 10% adapter dimers and require cumbersome adapter titration steps, the Swift RNA Library Kit produces <1% adapter dimers and maintains ligation efficiency at all supported input levels.
Swift’s Adaptase technology allows for minimal adapter dimers with NO need for adapter titration. Compared to leading RNA library kits which can produce libraries with > 10% adapter dimers and require cumbersome adapter titration steps, the Swift RNA Library Kit produces <1% adapter dimers and maintains ligation efficiency at all supported input levels.
Universal Human Reference Total RNA (Agilent 740000) was poly(A) enriched using the NEBNext poly(A) mRNA Magnetic Isolation Module (NEB E7490) before being processed through the Swift RNA Library Kit or processed following the supplier recommendations. Libraries were sequenced on a MiniSeq with 2 x 75 bp paired-end reads. Fastq files were downsampled to 2.2 million reads before analysis using STAR (mapping rate), rnaseqc (genes/trancsripts detected), or picard (duplication rate).
The Swift RNA Library Kit leverages an Indexing PCR step to complete the fully indexed adapter sequences, using primers that anneal to the truncated adapters added during the Adaptase and Ligation steps, to be fully compatible with Illumina sequencers. Swift supplies a variety of index configurations and strategies, including:
Single indexing
Dual indexing as either:
Combinatorial dual indexing, up to 768 combinations
Unique dual indexing, up to 96 unique dual indices
Available in 24- or 96-reaction kit sizes, the Swift RNA Library Kit is offered at scales to support evaluation and adoption.
In addition, the Swift RNA Library Kit protocol is readily automatable. A 10% overage volume of reagents is supplied to accommodate automation. Please contact our automation team at Automation@swiftbiosci.com if you require additional reagent overage volume, if you would like to learn about our custom packaging options, or if you would like to request the assistance of our field support to develop and qualify optimized automated scripts with liquid handling platforms routinely used in NGS library preparation.
